Section Ii Resolution and Recovery of Large Dna Fragments
نویسنده
چکیده
This section describes the application of agarose gel electrophoresis to both analytical and preparative separation of DNA fragments. Standard agarose gels separate DNA fragments from ∼0.5 to 25 kb, whereas pulsed-field agarose gels resolve molecules from ∼10 to >2000 kb. Descriptions of standard and pulsed-field agarose gel electrophoresis as well as parameters affecting resolution of large DNA fragments are presented in UNITS 2.5A and 2.5B. Three different protocols that employ agarose gels to prepare DNA fragments are described in UNIT 2.6. A general description of gels as electric circuits can be found in the introduction to this chapter.
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